FIG. 3.

Reactivity of sera from infected MyD88-deficient mice to B. hermsii. IgM antibodies (A and C) and IgG antibodies (B) against B. hermsii were detected in sera from mice sacrificed at 6 days of infection by Western blot analysis with sonicated B. hermsii (A and B) or with the lipoprotein-enriched n-butanol extract of B. hermsii, as described in Material and Methods. Uninfected control sera were from C57BL/6 and MyD88^−/− mice. GlpQ was detected with rabbit antisera (IgG). Arrows denote reactivity to an ∼17-kDa lipoprotein.