TABLE 2.

Frequency of B1b B-cell population in naïve MyD88-deficient mice^e

Genotype	Total no. of cells recovereda (106)	%B1a + B1b B cellsb	%B1a B cellsc	%B1b B cellsd
Wild type	4.0 ± 1.9	10.9 ± 1.6	6.5 ± 1.3	4.4 ± 0.8
MyD88−/−	5.7 ± 2.9	11.2 ± 1.6	7.6 ± 1.1	3.6 ± 0.7

^aB-cell populations in the peritoneal wash were quantified by FACS analysis as described in Materials and Methods. Values represent the average frequency of cell populations from nine wild-type mice and five MyD88-deficient mice. At least 20,000 events were analyzed to determine B-cell frequency in individual mice.

^bTotal B1 B-cell populations were characterized as IgM⁺ IgD⁺ CD11b^lo.

^cB1a B cells were characterized as IgM⁺ IgD⁺ CD11b^lo CD5⁺.

^dB1b B-cell populations (IgM⁺ IgD⁺ CD11b^lo CD5⁻) were calculated by subtracting the frequency of B1a B cells from the total IgM⁺ IgD⁺ CD11b^lo B-cell population in individual mice and are reported as the means ± standard deviations for each group.

^eSignificantly different values for wild-type and MyD88^−/− mice were not achieved in any category by Student's t test.