FIG. 3.
CPAF-specific immune response after primary genital C. muridarum challenge. HLA-DR4 tg, C57BL/6, and MHC-IIΔ/Δ animals (five mice/group) were challenged i.vag with 1,500 IFU of C. muridarum, and CPAF-specific cellular and humoral responses were analyzed at timed intervals. (A) Cytokine recall response. On day 14 after challenge, splenocytes were removed and cultured (106 cells/well) for 72 h with medium, CPAF, or the unrelated antigen HEL. IFN-γ production was measured by analyzing the culture supernatants by ELISA, and results are expressed as pg/ml of IFN-γ. (B) On days 14, 21, and 28, mice were bled and sera were analyzed for anti-CPAF total antibody (Ab) by ELISA. The 50% maximal binding was used to calculate titers. All results are expressed as means ± SD and are representative of two independent experiments.