TABLE 2.

Isolation of RASV strain from chicken tissues at various times following inoculation

Plasmida and no. of days after inoculation	Log10 colony count of RASV strain isolated per g of tissue (geometric mean ± SE)b
	Liver	Spleen	Bursa	Cecum
pYA3653
3	2.13 ± 2.17c	2.26 ± 2.24c	6.81 ± 0.63	8.42 ± 0.09
6	4.03 ± 1.77	5.57 ± 1.01	6.74 ± 0.63	8.58 ± 0.85
9	3.68 ± 2.04	6.24 ± 0.56	6.30 ± 0.88	8.89 ± 0.36
12	2.67d	4.43d	6.71 ± 0.36	9.94 ± 1.44
15	2.1d	2.37d	5.06 ± 0.27	7.54 ± 0.49
pYA3657
3	NA	NA	6.73 ± 1.1	8.56 ± 0.32
6	2.71 ± 0.17	4.81 ± 0.66	5.99 ± 0.21	8.48 ± 0.47
9	1.72 ± 1.58c	3.34 ± 2.90c	5.39 ± 1.04	9.20 ± 0.38
12	1.82d	2.05 ± 1.97c	5.75 ± 0.55	8.10 ± 0.84
15	3.82d	2.43 ± 2.30c	6.13 ± 0.30	8.68 ± 0.74
pYA3658
3	2.08 ± 1.80c	2.08 ± 2.93c	6.48 ± 0.52	8.03 ± 0.37
6	2.69 ± 2.69c	4.92 ± 1.41	5.75 ± 0.77	7.70 ± 0.11
9	3.54 ± 1.65	4.24 ± 3.70c	6.12 ± 1.28	9.55 ± 0.75
12	0.00 ± 0.00	3.72 ± 3.22c	5.77 ± 0.42	9.11 ± 0.14
15	4.33d	3.43 ± 3.22c	5.31 ± 0.69	9.19 ± 0.82

^aGroups of 15 chicks were inoculated on the day of hatching with Salmonella serovar Typhimurium χ8879 (ΔsptP1033::xylE ΔasdA16 ΔphoP233) harboring plasmid pYA3653, pYA3657, or pYA3658.

^bObtained from three chickens. NA, data not available.

^cOne chick had <10 CFU/g of tissue.

^dTwo chicks had <10 CFU/g of tissue.