FIG. 4.

Allergic conjunctival and pulmonary inflammation following RW sensitization and challenge in IL-10-deficient mice. Five to eight IL-10-deficient mice were treated with PBS, RW, PCF, or RW and PCF. A. Five conjunctival sections per mouse per treatment group were counted to determine eosinophil infiltration 72 h postallergen challenge. RW sensitization and challenge in IL-10-deficient animals led to increased eosinophil infiltrates in the conjunctiva compared with all other groups (*, P < 0.05). Mice treated with PCF during RW sensitization had a reduced eosinophilic ocular infiltration after RW challenge compared with animals given RW alone. B. IL-10-deficient mice given RW sensitization had a significant increase in lung inflammatory scores compared to mice sensitized with PBS or PCF treatment alone post-RW challenge (***, P < 0.001). Mice treated with PCF during RW sensitization had a reduced lung inflammatory score after RW challenge compared with animals given RW alone.