FIG. 1.

Up-regulation of IFN-γR expression by C. psittaci is TLR dependent. HEK 293 cells stably transfected with TLR2, TLR4/MD-2, or the empty pcDNA expression vector were cultivated in complete medium, treated with IL-1β (100 ng/ml), or infected with C. psittaci for 24 h and stained with antibody to IFN-γR and PE-conjugated secondary antibody. The mean fluorescent intensities (MFIs) were determined using flow cytometry. The results are representative of several independent experiments with similar results (MFIs ± standard deviations).