FIG. 3.

Dependency of IFN-γR expression up-regulation on NF-κB transactivation. (Top) HeLa 229 cells were cultivated in complete medium, treated with IL-1β (100 ng/ml), or infected with C. psittaci for 1 h. Nuclei were harvested and subjected to flow cytometric analysis of intranuclear NF-κB present with an antibody to the p65 subunit of NF-κB. (Bottom) HeLa 229 cells were cultivated in complete medium, treated with IL-1β (100 ng/ml), or infected with C. psittaci, alone or in combination with PSI (3 μM) for 12 h. The cells were harvested, and IFN-γR expression was determined by flow cytometric analysis using an anti-receptor antibody. The results from one of several experiments with similar results are presented (mean fluorescent intensities [MFIs] ± standard deviations).