FIG. 6.

Results of the reverse experiment to Fig. 5 but still with the modified inoculation procedure for mouse colonization. Mice were inoculated with H. pylori two times, with the second inoculation 2 days after the first. Each time, the inoculum contained 2 × 10⁸ dead napA mutant cells and 4 h later 2 × 10⁸ live test cells (i.e., either live wild-type [WT] cells or live napA mutant cells). Colonization was examined 3 weeks after the first inoculation. Data collection and analysis were done as described in the legend to Fig. 4. According to Wilcoxon rank test analysis, there is no significant difference between the wild type and the napA mutant in mouse colonization ability under these conditions.