FIG. 9.

VacA-positive H. pylori cells recruit and retain active Rab7 to their intracellular compartment. AGS cells were transfected with GFP-Rab7 and then infected with H. pylori for 24 h. GFP-Rab7 mobility was then estimated by FRAP. Panel A shows a representative quantitation and nonlinear regression fitted curve of the fluorescent recovery of Rab7 in endosomes from uninfected cells (blue line and triangles) and the H. pylori compartment (red line and squares). The summary of the fractional recovery of Rab7 in endosomes (light gray bar) and bacterial vacuoles (dark gray bar) from invaded cells is shown in panel B. Data are mean ± standard error of 10 cells.