FIG. 1.
HP1512 transcript is significantly down-regulated in the presence of nickel as measured by real-time qPCR. H. pylori 26695 was grown in BBF supplemented with 0, 1, 5 or 10 μM NiCl2. The transcript level was determined relative to the transcript levels from cultures with no additional nickel added. The values (changes [n-fold]) represent the averages from three independent assays. As the nickel concentration increased, the HP1512 transcript level decreased in a stepwise fashion relative to the HP1512 transcript level for cells grown in unsupplemented BBF. With the addition of 10 μM NiCl2, the HP1512 transcript level decreased approximately 43-fold.