FIG. 3.

Growth curve of the HP1512::cat (1512::cat) mutant compared to the wild type. Insertional inactivation of the HP1512 gene resulted in reduced growth relative to the wild-type H. pylori 26695 strain cultured in unsupplemented BBF. The optical density at 600 nm (indicated by the lines) was monitored in three independent cultures. At each time point, the number of CFU per milliliter was determined by combining the independent cultures in equal volumes and plating for viable colonies; thus, each bar represents the CFU/ml from a single pooled culture.