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. 2006 Oct 9;74(12):6811–6820. doi: 10.1128/IAI.01188-06

TABLE 1.

Oligonucleotide primers used in this study

Locus (gene) Direction of primera Primerb Sequence (5′→3′)
HP1512 (frpB4) F HP1512.1 GTTTGTCTCTTGTATTGTCG
R HP1512.2 CCACATATTGCTTCTTAAAG
HP1338 (nikR) F NikR-1 ACTAGTCCATGGATACACCCAATAAAGAC
R N4_NotI GCGGCCGCCGCTCAACAGTACCCTATCTATCCC
HP1511-1512 intergenic region F IG1511-12-1 TCACGAACAGTGCTTGATTCAG
R HP1511-12-2 CGATACGAAATTGATTTCTAAGC
HP1512 (frpB4) F HP1512RTF TCTAGCTGCAACGACTGGGAATGT
R HP1512RTR AGCGCGAGAGCCAGGTTAGGATA
HP1400 (fecA3) F HP1400RTF TTGAGCGATCGCATTGAAGCTTGG
R HP1400RTR AAGCCAATGTTGGTTGAGGGCATC
HP1010 (ppk) F HP1010RTF GCGCGTTAGTCGTTTATGGCGTTT
R HP1010RTR AGCGCTCAAAGGGTTGTAATTGCC
HP1077 (nixA) F HP1077RTF CGTTTGATGCGGATCACATCGCTT
R HP1077RTR TCTTCTAGCATCGGCGTGTGTTCT
HP1338 (nikR) F HP1338RTF CGCCGTGCTTGTGGTGATTTATGA
R HP1338RTR CCATGTGAATGTGCGTGGTGCATA
HP0073 (ureA) F HP0073RTF CTGAATTGATGCAAGAAGGGCGCA
R HP0073RTR CCACTTCATGGATCATGCTTGCCA
a

F, forward; R, reverse.

b

Primers HP1512.1 and HP1512.2 were used for construction of the HP1512::cat mutants. Primers NikR-1 and N4_NotI were used for expression of recombinant NikR in pET30a. Primers used for real-time PCR are designated with RTF (real-time forward) and RTR (real-time reverse) in the primer name. HP1010 (ppk) served as a housekeeping gene for the quantitative real-time PCR experiments.