TABLE 1.
Insertion sites of pMarGent mutants (grouped by infectivity phenotype)
| Infectivitya | Clone | Replicon/positionb | Insertion site(s)c | Description |
|---|---|---|---|---|
| + | MG004 | Chrom./108542 | BB0110 (108307-109671) | Hypothetical protein |
| + | MG005 | cp32-6/24624 | BBM36 (24619-25044) | Borrelia plasmid protein B (bppB) (putative) |
| + | MG006 | lp36/17582 | BBK25 (17565-16966) promoter | Transposase-like protein (putative), authentic frameshift |
| + | MG007 | Chrom./534145 | Intergenic; BB0524 (534301-535115) and BB0523 (533980-534072) | BB0524 possible myoinositol-1 (or 4)- monophosphatase (putative); BB0523 is a hypothetical protein |
| + | MG010 | cp32-3/27222 | BBS41 (26708-27298) | Outer surface protein G (erpG) |
| + | MG011 | lp28-3/4210 | BBH09 (7728-3892) | Hypothetical protein |
| + | MG012 | lp28-1/4515 | BBF10 (4488-4985) | Hypothetical protein |
| + | MG016 | cp32-8/25599 | BBL38 (25951-25175) | Borrelia plasmid protein C (bppC) (putative) |
| + | MG017 | lp38/33790 | Intergenic; BBJ45 (32498-33220) and BBJ46 (34668-34372) | BBJ45 and BBJ46 are hypothetical proteins |
| + | MG018 | cp32-4/24618 | BBR37 (24210-24635) | Borrelia plasmid protein B (bppB) (putative) |
| + | MG020 | cp26/23390 | BBB28 (23255-24499) | Hypothetical protein |
| + | MG025 | lp21/7829 | Intergenic; BBU05 (2868-3656) and BBU06 (14633-15235) | Within central 63-bp repeat region of lp21 |
| + | MG026 | lp36/23615 | BBK37 (23953-22910) | Homolog of immunogenic protein P37 |
| + | MG027 | cp32-4/7055 | BBR11 (6711-7823) | Hypothetical protein |
| + | MG028 | cp32-4/17887 | BBR29 (18664-17573) | Conserved hypothetical protein |
| + | MG029 | lp28-1/5088 | Intergenic; BBF10 (4488-4985) and BBF11 (5435-5542) | BBF10 and BBF11 are hypothetical proteins |
| + | MG030 | cp26/25676 | BBB29 (24825-26453) | Phosphotransferase system enzyme, maltose- and glucose-specific IIABC component (malX) |
| + | MG032 | lp17/9413 | Intergenic; BBD14 (8269-9381) and BBD15 (10015-9593) | BBD14 is a conserved hypothetical protein and BBD15 is a hypothetical protein |
| +/− (lp28-1−) | MG008 | Chrom./660583 | BB0629 (661524-659644) | Phosphotransferase system enzyme II (fruA-2) |
| +/− (lp28-1−) | MG015 | lp38/25996 | BBJ34 (26407-25337) | Hypothetical protein |
| +/− (lp28-1−) | MG021 | lp28-2/20360 | BBG24 (22310-19620) | Hypothetical protein |
| +/− (lp28-1−) | MG022 | lp25/21344 | Intergenic; BBE29 (19697-20886) and BBE30 (21558-21704) | BBE29, putative adenine-specific methyltransferase, authentic frameshift; BBE30, hypothetical protein |
| +/− (lp28-1−) | MG023 | lp21/6922 | Intergenic; BBU05 (2868-3656) and BBU06 (14633-15235) | Within central 63-bp repeat region of lp21 |
| +/−(lp28-1−) | MG031 | cp26/12701 | BBB16 (12014-13606) | Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (oppAIV) |
| +/− (lp28-1+) | MG009 | Chrom./786112 | BB0743 (785057-786754) | Hypothetical protein |
| +/− (lp28-1+) | MG013 | Not determined | ||
| +/− (lp28-1+) | MG014 | Chrom./841814 | BB0797 (839244-841832) | DNA mismatch repair protein (mutS) |
| +/− (lp28-1+) | MG019 | cp9/5623 | BBC08 (5534-5980) | Conserved hypothetical protein |
| +/− (lp28-1+) | MG033 | cp32-7/22694 | BBO35 (22755-22627) | Hypothetical protein |
| − | MG001 | Not determined | ||
| − | MG002 | cp26/14424 | BBB17 (15107-13893) | IMP dehydrogenase (guaB) |
| − (lp25−) | MG003 | cp26/11461 | Intergenic between BBB14 (11417-10920) and BBB15 (11636-11740) | BBB14 and BBB15 are hypothetical proteins |
| − | MG024 | Chrom./226987 | BB0221 (227836-226610) | Flagellar motor switch protein (fliG-1) |
a
+, culture positive at all sites; +/−, culture positive at some sites; −, culture negative at all sites.
b
Chrom., chromosome.
c
Position of gene given in parentheses. Reverse coordinates indicate that the gene is encoded on the negative strand.