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. 2006 Oct 2;74(12):6690–6699. doi: 10.1128/IAI.00993-06

TABLE 2.

Infectivities of noninfectious and lp28-1+ attenuated mutantsa

Strain and inoculum No. of tissue samples infected/no. tested
No. of mice infected/no. tested No. of mice reactive/no. testedc
Ear Bladder Heart Joint All sitesb
5A18 NP1 (parental strain)
    102 0/4 0/4 0/4 0/4 0/16 0/4 0/4
    104 3/4 4/4 4/4 4/4 15/16 4/4 4/4
    106 4/4 4/4 4/4 4/4 16/16 4/4 4/4
    Single mouse (104) 1/1 1/1 1/1 1/1 4/4 1/1 1/1
        Total 8/13 9/13 9/13 9/13 35/52 9/13 9/13
MG001 (insertion site ND; no plasmids lost)
    102 0/4 0/4 0/4 0/4 0/16 0/4 0/4
    104 0/4 0/4 0/4 0/4 0/16 0/4 1/4
    106 0/4 0/4 0/4 0/4 0/16 0/4 2/4
    Single mouse (104) 0/1 0/1 0/1 0/1 0/4 0/1 1/1
        Total 0/13 0/13 0/13 0/13 0/52 0/13 4/13
MG002 (guaB; cp9− cp32-3−)
    102 0/4 0/4 0/4 0/4 0/16 0/4 0/4
    104 0/4 0/4 0/4 0/4 0/16 0/4 0/4
    106 0/4 0/4 0/4 0/4 0/16 0/4 1/4
    Single mouse (104) 0/1 0/1 0/1 0/1 0/4 0/1 0/1
        Total 0/13 0/13 0/13 0/13 0/52 0/13 1/13
MG024 (fliG-1; no plasmids lost)
    102 0/4 0/4 0/4 0/4 0/16 0/4 0/4
    104 0/4 0/4 0/4 0/4 0/16 0/4 0/4
    106 0/4 0/4 0/4 0/4 0/16 0/4 0/4
    Single mouse (104) 0/1 0/1 0/1 0/1 0/4 0/1 0/1
        Total 0/13 0/13 0/13 0/13 0/52 0/13 0/13
MG009 (BB0743; cp9−)
    102 0/4 1/4 1/4 2/4 4/16 2/4 2/4
    104 1/4 4/4 4/4 3/4 12/16 4/4 4/4
    106 0/4 4/4 3/4 4/4 11/16 4/4 4/4
    Single mouse (104) 0/1 1/1 1/1 1/1 3/4 1/1 1/1
        Total 1/13 10/13 9/13 10/13 30/52 11/13 11/13
MG013 (insertion site ND; cp9−)
    102 0/4 1/4 0/4 1/4 2/16 1/4 0/4
    104 0/4 2/4 1/4 3/4 6/16 3/4 4/4
    106 2/4 4/4 0/4 4/4 10/16 4/4 4/4
    Single mouse (104) 0/1 1/1 0/1 1/1 2/4 1/1 1/1
        Total 2/13 8/13 1/13 9/13 20/52 9/13 9/13
MG014 (mutS; cp9−, cp32-6−)
    102 0/4 0/4 0/4 0/4 0/16 0/4 0/4
    104 2/4 3/4 2/4 3/4 10/16 3/4 3/4
    106 1/4 4/4 3/4 4/4 12/16 4/4 4/4
    Single mouse (104) 0/1 1/1 1/1 1/1 3/4 1/1 1/1
        Total 3/13 8/13 6/13 8/13 25/52 8/13 8/13
MG019 (BBC08; lp38−)
    102 0/4 0/4 0/4 0/4 0/16 0/4 0/4
    104 0/4 1/4 0/4 1/4 2/16 1/4 0/4
    106 0/4 2/4 1/4 0/4 3/16 2/4 2/4
    Single mouse (104) 0/1 0/1 1/1 1/1 2/4 1/1 1/1
        Total 0/13 3/13 2/13 2/13 7/52 4/13 3/13
a

Transposon insertion sites and plasmids missing (in addition to lp28-4 and lp56, which are absent in the parental strain 5A18 NP1) are indicated for each clone. Single mouse, results from single mouse inoculation study (see Table 1). ND, not determined.

b

χ2 analysis found the combined 104 and 106 inoculum data for each of the mutants to be statistically different from those for the 5A18 NP1 control (P < 0.05).

c

Reactive mice are those for which a significant serological response was demonstrated in a VlsE ELISA.