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TLR2 and TLR4 ligands induce IκBα phosphorylation and degradation in skeletal muscle cells. Immunoblot analysis of phosphorylated IκBα (P-IκB) and total IκBα (IκB) in C2C12 myotubes transfected with a dominant-negative IKKβ plasmid vector or an empty control vector containing the same plasmid backbone and stimulated with LPS (1 μg/ml) or PGN (10 μg/ml).
