TABLE 2.
DC numbers and cytokine concentrations in cases and controls
| Parametera | Median (25th-75th percentile)b
|
||
|---|---|---|---|
| Acute sample (n = 33) | Convalescent sample (n = 33) | Control sample (n = 33) | |
| CD1c+ mDCs (103/ml) | 9.5 (5.8-18.9) | 15 (8.8-26.5) | 13 (9.3-18.5) |
| CD123+ pDCs (103/ml) | 4.9 (2.3-13.1) | 9.4 (5.3-20.8) | 9.3 (4.6-13.8) |
| BDCA3+ mDCs (103/ml) | 5.0* (2.1-9.0) | 6.6* (2.1-30.0) | 1.2 (0.6-2.0) |
| CD14+ monocytes (106/ml) | 0.39* (0.19-0.73) | 0.51* (0.26-0.93) | 0.10 (0.08-0.14) |
| CD1c+ mDCs (GMFI HLA DR) | 24.3* (17.0-34.2) | 41.4 (28.3-49.6) | 36.5 (30.3-44.3) |
| CD123+ pDCs (GMFI HLA DR) | 12.6 (6.6-21.1) | 15.1 (10.8-19.3) | 16.9 (11.5-20.1) |
| BDCA3+ mDCs (GMFI HLA DR) | 9.2* (5.1-15.0) | 24.1 (15.8-37.5) | 19.7 (13.4-28.8) |
| CD14+ monocytes (GMFI HLA DR) | 13.6* (10.1-24.1) | 27.5 (17.8-52.0) | 31.5 (28.3-36.5) |
| IL-10 (pg/ml) | 1,731* (538-2,533) | 69 (21-140) | 44 (17-82) |
| TNF-α (pg/ml) | 35* (11-82) | 0 | 0 |
| IL-12 (pg/ml) | 183* (91-274) | 0 | 0 |
a
GMFI HLA DR, Geometric mean fluorescence intensity of HLA DR expressed on monocytes or DC subsets. Of note, the fluorescence intensity scale on an Epics II flow cytometer from Beckman Coulter begins with 0.1 rather than 1.
b
*, P < 0.001 compared to control (Mann-Whitney U test).