TABLE 1.
Oligonucleotide primers used in this study
| Primer | Sequence (5′→3′) |
|---|---|
| frpB4-F4 | AGCCGTCTCTTAAGGGTAAC |
| frpB4-R-T7a | ctaatacgactcactatagggagaTCGCTATTGCTTGGATCTTG |
| frpB4-DFP-for | TGCTTGATTCAGCCGCTCAG |
| frpB4-DFP-revb | TGCTAGCGACAATACAAGAG |
| fecA3-F3 | GATTACCGCGCCTAAGAGTT |
| fecA3-R4-T7a | ctaatacgactcactatagggagaCTGCCTCCACCCTTGATCAC |
| fecA3-DFP-for | GCGTCAAAGAGTGTCTTGTG |
| fecA3-DFP-revb | TCCTTAGCGAACAAAGACTC |
| Hp1339-F | AGCTTTGTGGTTTGCGATTG |
| Hp1339-R-T7a | ctaatacgactcactatagggagaGTGGGAATCGCCACAGCAAG |
| Hp1340-F | AGCATCAGAAGAGGCGATGG |
| Hp1340-R-T7a | ctaatacgactcactatagggagaCTGAGCTTGCGTGGAGATGG |
| Hp1341-F | AATGCTGAGTCGGCTAAACC |
| Hp1341-R-T7a | ctaatacgactcactatagggagaGTCCGTAACGCTCCCATCAG |
a
Primer contains a 5′ extension with T7 promoter sequence (in lowercase letters) for the creation of an antisense RNA probe.
b
Primer is digoxigenin labeled.