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Recognition of rCaf1 and cpCaf1 by a protective monoclonal anti-Caf1 antibody. Plates were coated with the dose range shown of rCaf1, cpCaf1, or V antigen of Y. pestis as a negative control; incubated with 0.64 μg of the protective monoclonal antibody F1-04-A-F1/ml; and developed by ELISA as described in Materials and Methods. The results are plotted as the mean absorbance at 405 nm for duplicate wells.
