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Purification of recombinant Map-PtpA
| Purification step (fraction) | Vol (ml) | Total activity (U)a | Total protein (mg) | Sp act (U/mg of protein) | Yield (%) | Fold increase |
|---|---|---|---|---|---|---|
| Starting material | 10 | 23.6 | 1.77 | 1.33 | 100 | 1 |
| Washing with 20 mM imidazole | 3 | 1.65 | 0.45 | 1.22 | 6.99 | 0.91 |
| Elution with 250 mM imidazole | 0.8 | 22.9 | 0.15 | 153 | 97 | 115 |
The phosphatase activity was measured by using pNPP as a substrate. One unit of phosphatase activity is defined as the formation of 1 μmol of p-nitrophenol per min.
