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Quantification of biofilm formation by the PAK, lecA mutant, lecB mutant, complemented lecB mutant (lecB+), and PAK-NP strains in a crystal violet microtiter plate assay after 24 h. Absorbance was measured at 600 nm. The means ± standard deviation (error bars) were determined from at least three independent experiments. The difference between the parent strain and lecB mutant was statistically significant (P < 0.05).
