FIG. 7.

MglA-regulated genes contribute to Francisella virulence in mice. Mice were infected subcutaneously with equal amounts of mutant and wild-type Francisella cells. At 2 days postinfection, spleens were taken for counts, and the competitive index was calculated. (A) Competitive indices of pdpB and cds2 FPI mutants. A spontaneous streptomycin-resistant mglA mutant (see Materials and Methods for a description) and the pdpA mutants were included as controls. (B) Competitive indices of FTT0612, FTT0989, oppB, and FTT1209c mutants. At least five mice were used to calculate the CI value for each mutant strain. Each spot represents the CI value from an individual mouse, and bars represent the geometric means. Significance was calculated by comparing the log of the CI value to 0. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (compared with 0).