FIG. 6.

Anti-CXCL16 treatment inhibits IFN-γ production by spleen lymphocytes in response to primary serovar Enteritidis infection. Mice were pretreated with 500 μg of protein A-purified NRIgG or anti-CXCL16 the evening prior to primary infection with serovar Enteritidis and the level of intracellular IFN-γ was measured in the lymphocyte (CD3⁺), NK cell (DX5⁺), macrophage (F4/80⁺) and neutrophil (Ly6G⁺) populations of the spleen. (A) Representative dot plots. The upper-right quadrant shows the percentage values for double-positive cells. (B) Percentages of IFN-γ⁺ cells in the various splenic populations. The data represent means ± the SEM (n = 4). **, Significantly different from the NRIgG value at P < 0.01 (Student t test).