FIG. 6.
Caspase-3 activation in EGFP-ExoS transfected HeLa cells is dependent on JNK1 activation and cytochrome c release. (A and B) HeLa/pcDNA3 (vector) or HeLa/DN JNK1 cells (A) and HeLa/Neo vector (vector) or HeLa/Bcl-xL cells (B) were transfected with the EGFP-ExoS construct and subjected to caspase-3 activation assay with Red-DEVD-FMK as described above. As a control for apoptosis induction, STS (2 μM) was used to treat the indicated cells for 3 h. The data are means ± the SD from three replicates from one transfection experiment. Significant differences between HeLa/pcDNA3 (vector) or HeLa/DN JNK1 cells (A) or between HeLa/Neo vector (vector) and HeLa/Bcl-xL cells (B) are indicated (*, P < 0.05; **, P < 0.01; ***, P < 0.001).