FIG. 5.

Coenzyme M restores epoxypropane degradation activity in BES-treated X. autotrophicus cell suspensions. Cells (0.12 mg protein) were preincubated with 2 μmol epoxypropane in the absence or presence of BES for 300 min, as shown in Fig. 4. The cells were then washed and resuspended in fresh medium, and epoxypropane degradation activity was assayed as described in Materials and Methods. The solid symbols show the results of assays performed with cells preincubated without BES, while the open symbols show the results of assays performed with cells preincubated with 5 mM BES. Triangles, assays conducted without supplemental CoM; squares, assays conducted in the presence of 5 mM CoM.