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. 2006 Sep 22;188(23):8062–8069. doi: 10.1128/JB.00947-06

FIG. 6.

FIG. 6.

BES inactivation of epoxide isomerase activity in cell extracts of X. autotrophicus and restoration of epoxide isomerase activity in BES-treated cell extracts by addition of purified 2-KPCC. Cell extracts were prepared and pretreated with or without 5 mM BES present, and epoxide isomerase assays were conducted as described in Materials and Methods. The data show the time course for (R)-epoxypropane isomerization to acetone, a reaction requiring active EaCoMT, R-HPCDH, and 2-KPCC. (A) Activity in the absence of purified enzymes. Symbols: •, no BES present in pretreatment; ○, 5 mM BES present in pretreatment. (B) Activity in BES-pretreated extracts in the presence of purified enzymes. Symbols: •, no purified enzyme added; ▾, purified EaCoMT added; ▵, purified R-HPCDH added; ○, purified 2-KPCC added.