TABLE 2.
Genes of C. glutamicum whose average mRNA ratio was altered ≥2.8-fold (P ≤ 0.05) in myo-inositol-grown cells compared to that in glucose-grown cells in at least three independent cultivations
| NCBIa designation | Open reading frame | Annotation | Gene | mRNA ratiob
|
|
|---|---|---|---|---|---|
| Inositol vs Glucose | BHI vs CGXII | ||||
| NCgl0029 | Cgl0030 | ABC transporter/periplasmic d-ribose-binding protein | rbsB | 0.19 | 0.38 |
| NCgl0155 | Cgl0158 | myo-Inositol catabolism, carbohydrate kinase | iolC | 4.94 | 3.07 |
| NCgl0157 | Cgl0160 | myo-Inositol catabolism, aldehyde dehydrogenase | iolA | 16.65 | 7.16 |
| NCgl0158 | Cgl0161 | myo-Inositol catabolism | iolB | 16.55 | 15.04 |
| NCgl0159 | Cgl0162 | myo-Inositol catabolism, thiamine pyrophosphate-requiring enzyme | iolD | 12.01 | 4.66 |
| NCgl0160 | Cgl0163 | 2-Keto-myo-inositol dehydratase | iolE | 18.64 | 13.93 |
| NCgl0161 | Cgl0164 | myo-Inositol dehydrogenase, oxidoreductase | iolG | 16.55 | 7.38 |
| NCgl0162 | Cgl0165 | myo-Inositol catabolism, isomerases/epimerase | iolH | 8.32 | 3.69 |
| NCgl0163 | Cgl0166 | Efflux carrier, major facilitator superfamily; MFS1 | 17.33 | 2.33 | |
| NCgl0164 | Cgl0167 | myo-Inositol dehydrogenase, oxidoreductase | oxi1 | 2.85 | 0.88 |
| NCgl0167 | Cgl0170 | Transcriptional regulator, LacI type; LacI1 | 7.93 | 4.07 | |
| NCgl0168 | Cgl0171 | Putative oxidoreductase dehydrogenases | oxiB | 9.37 | 5.14 |
| NCgl0178 | Cgl0181 | myo-Inositol transporter | iolT1 | 12.86 | 7.17 |
| NCgl0697 | Cgl0727 | Trehalose/maltose-binding protein; malE | 0.25 | 2.53 | |
| NCgl0916 | Cgl0954 | γ-Glutamyltransferase; ggt | 0.34 | 1.34 | |
| NCgl0933 | Cgl0972 | Porin | porB | 0.25 | 2.52 |
| NCgl1368 | Cgl1423 | Putative acetyltransferase | 0.21 | 3.36 | |
| NCgl1917 | Cgl1992 | ABC transporter/oligopeptide permease; oppC | 0.29 | 2.63 | |
| NCgl2477 | Cgl2566 | Succinyl-CoA synthetase (beta chain) | sucC | 0.23 | 1.53 |
| NCgl2865 | Cgl2967 | Secreted multicopper oxidase; cumA | 4.40 | 1.29 | |
| NCgl2894 | Cgl2996 | myo-Inositol-1-phosphate synthase | ips | 0.09 | 0.17 |
| NCgl2904 | Cgl3007 | Malic enzyme | mez | 0.33 | 3.27 |
| NCgl2951 | Cgl3056 | Hydroxyquinol 1,2-dioxygenase; catA | 9.48 | 2.12 | |
| NCgl2952 | Cgl3057 | Iron-containing alcohol dehydrogenase, oxidoreductase; adh1 | 7.56 | 7.27 | |
| NCgl2953 | Cgl3058 | myo-Inositol transporter | iolT2 | 13.94 | 6.19 |
| NCgl2955 | Cgl3060 | myo-Inositol dehydrogenase | oxiC | 18.83 | 9.85 |
| NCgl2956 | Cgl3061 | myo-Inositol catabolism, sugar phosphate isomerase/epimerase | 21.02 | 8.67 | |
| NCgl2957 | Cgl3062 | myo-Inositol dehydrogenase | oxiD | 26.83 | 9.53 |
| NCgl2958 | Cgl3063 | myo-Inositol dehydrogenase | oxiE | 12.54 | 3.04 |
| NCgl2959 | Cgl3064 | Secreted phosphoesterase | 4.34 | 0.6 | |
| NCgl2961 | Cgl3066 | Proline/ectoine carrier | proP | 0.30 | 0.83 |
a
Numbers for the corresponding open reading frames of the C. glutamicum genome NC_003450 are given. NCBI, National Center for Biotechnology Information.
b
The first column gives the mRNA ratio for genes of C. glutamicum ATCC 13032 grown on myo-inositol to that of cells grown on glucose. The far right column gives the ratios for BHI- versus CGXII-glucose grown cells, and data are limited to those genes where myo-inositol utilization already revealed altered mRNA levels.