TABLE 1.
Plasmid (genotype) | Avg retention rate (%) at no. of generations
|
|||
---|---|---|---|---|
0 | 20 | 40 | 60 | |
pLGUS (parA orf4) | 100 | 100 | 100 | 100 |
pOR302 (parA Δorf4) | 100 | 96.9 | 22.8 | 6.4 |
pOR303 (ΔparA Δorf4) | 91.5 | 78.7 | 13.8 | 5.2 |
The plasmid stability was analyzed as described in Materials and Methods. Two parallel experiments were performed for each plasmid, and the percentages of recovered colonies that contain plasmid are shown.