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. 2006 Sep 29;188(23):8013–8021. doi: 10.1128/JB.01160-06

FIG. 4.

FIG. 4.

(A) Northern blot membrane probed with a mucD-specific antisense RNA. The lanes contain 1.5 μg total RNA isolated from cultures grown to an OD600 of 1.0 from A. vinelandii wtC15 at 28°C (lane 1), P. aeruginosa Pa8821 at 37°C (lane 2), P. aeruginosa Pa8822 at 37°C (lane 3), P. aeruginosa Pa8830 at 37°C (lane 4), P. syringae PG4180 at 18°C (lane 5) or 28°C (lane 6), and P. syringae PG4180.muc at 18°C (lane 7) or 28°C (lane 8). The obtained signal, slightly above the 16S rRNA band, is marked with the estimated size. (B) Methylene blue-stained membrane prior to Northern blot hybridization for transcript size estimation, control of total RNA quantity, and successful Northern transfer. The same region of the membrane as that shown in panel A is shown. Distinct bands in lanes 1 to 8 represent those of the 23S (upper) and 16S rRNA (lower) or of the RNA size standard (3.0 μg) in lane M.