TABLE 2.
Oligonucleotide primers used in this study
| Oligonucleotide primer | Nucleotide sequencea (5′ to 3′) |
|---|---|
| alg-Operon_fwd | TGCTCTAGAGGCGCGCTGTTCAAGCAGTTCGAT |
| alg-Operon_rev | CCCAAGCTTCGATGATGGAGAAATTGCGG |
| alg_seq1 | TGACGCTCGCAATGTCTTC |
| alg_seq2 | AACACGCCGTTGGCCATA |
| alg_seq3 | CAGGTGAAGTTCAAGGCCG |
| alg_seq4 | TTCAGTACCTGCGCCCAAG |
| algT_fwd | CGGATCCCTTAACGAGGAGTGTTCATG |
| algT_revT7 | TAATACGACTCACTATAGGGAGGACGGTACCAACAGGACACTG |
| mucA_fwd | TCCGCGGTACTGGATAACGAAGC |
| mucA_revT7 | TAATACGACTCACTATAGGGAGGACGCCGTTGGCCATAGGG |
| mucB_fwd | TTCTGCGTGTTGACGGGCTT |
| mucB_revT7 | TAATACGACTCACTATAGGGAGGACATCAGATGGGTAACGG |
| mucB2_fwd | TGATGTATGGCGACGGTCTGGC |
| mucB2_revT7 | TAATACGACTCACTATAGGGAGGGTCACCATCATGTCGCCC |
| mucD_fwd | CGAATTTCTCGAGCGCAGCATGC |
| mucD_revT7 | TAATACGACTCACTATAGGGAGGGGAGCGGGTAAATATCTGCG |
| mucD_fwd_deg | TCGCCGGCGGTGGTCAATATCAGTAC |
| mucD_revT7_deg | TAATACGACTCACTATAGGGAGGCGAACCGATGGCCAGGACCCATTC |
| algD_fwd | GCGTATCAGCATATTTGGTTTGGG |
| algD_revT7 | TAATACGACTCACTATAGGGAGGCGTTGCAGGTGTACTTGATCA |
| mucD-Ps-3_pe | GAGCACAGCGGCAATCAGTG |
a
Restriction sites incorporated in primers are underlined; TCTAGA, XbaI; AAGCTT, HindIII. T7 RNA polymerase promoter sequences incorporated in primers are italics