TABLE 1.

Plasmids used in this study

Plasmid	Descriptiona	Phenotypeb	Source or reference
S. coelicolor
pIJ486	ter neo tsr ori pIJ101 rep pIJ101 MCS	Thior	14
pIJ486BB	ter neo tsr ori pIJ101 rep pIJ101 MCS bldB lacZα aac(3)IV ori pUC18	Aprr/Thior	This work
pRA1	lacZα aac(3)IV ori pUC18 oriT (RK2) int ϕC31 φC31 attP MCS bldB + 304-bp promoter region	Aprr	7
E. coli
pT18NHB	bla ori colE1 f1 origin T18 MCS 0.32-kb KpnI amplicon from pBB801 containing bldB inserted into pT18	Ampr	6
pT25NHB	cat ori p15A T25 MCS 0.32-kb KpnI amplicon from pBB801 containing bldB inserted into pT25	Chlr	7

^aMCS, multiple cloning site.

^bAntibiotic resistance markers are apramycin (Apr^r), ampicillin (Amp^r), thiostrepton (Thio^r), and chloramphenicol (Chl^r).