FIG. 3.

Far-UV CD spectroscopy of SsuE and SsuD. The spectrum of SsuE (solid line) was obtained with 2.4 μM SsuE in 25 mM phosphate buffer (pH 7.5) at 25°C. The SsuD spectrum (dotted line) was obtained with 1.2 μM SsuD in 25 mM phosphate buffer (pH 7.5) at 25°C. The SsuE/SsuD spectrum (dashed line) was obtained with 2.4 μM of SsuE and 1.2 μM SsuD. The additive spectrum was from individual spectra of SsuE and SsuD (dashed line). Measurements were taken in 0.1-nm increments from 300 to 185 nm in a 0.1-cm-path-length cuvette. Each spectrum is the average of four scans; smoothing of the data was performed using the default parameters within the Jasco J-720 software.