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Reaction progress of CO2 hydration with intact and ruptured carboxysomes. Samples of carboxysomes were identical, except that the ruptured sample had been subjected to one round of freeze-thaw treatment before the assay was performed as described in Materials and Methods. The amount of carboxysome protein used in these assays corresponded to 0.25 μM CsoSCA (□, intact carboxysomes; ▪, broken carboxysomes).
