TABLE 1.
Metal dependence of recombinantly expressed carboxysomal carbonic anhydrase rCsoSCA from H. neapolitanus
| Metal added (1 mM) | Relative activity (%)a |
|---|---|
| None | 100 |
| Zn2+ | 92.8 |
| Co2+ | 101.8 |
| Fe2+ | 98.1 |
| Mg2+ | 98.4 |
| Chelex-treated buffer | 28.4 |
| Chelex-treated buffer + Zn2+ | 84.6 |
| Chelex-treated buffer + Co2+ | 25.9 |
| Chelex-treated buffer + Fe2+ | 33.4 |
| Chelex-treated buffer + Mg2+ | 26.7 |
a
CO2 hydration by rCsoSCA (0.5 μM) was measured by stopped-flow spectrophotometry at pH 8 (buffer/indicator pair, TAPS/m-cresol purple). Values are averages of three determinations with maximum variations of less than 12%.