TABLE 3.
Fraction | Kinetic constant value for:
|
|||
---|---|---|---|---|
Hydration reactiona
|
Dehydration reactionb
|
|||
kcat (s−1) | Km (mM) | kcat (s−1) | Km (mM) | |
rCsoSCA | (8.9 ± 0.5) × 104 | 3.2 (± 0.4) | (4.6 ± 1.2) × 104 | 9.3 (± 2.1) |
Broken carboxysomes | (6.5 ± 0.9) × 104 | 3.6 (± 0.7) | (3.1 ± 0.8) × 104 | 10.1 (± 3.2) |
Intact carboxysomes | (1.8 ± 0.2) × 104 | 12.2 (± 2.3) | ND | ND |
The hydration reaction was performed at pH 8.0.
The dehydration reaction was performed at pH 7.0. ND, not determined.