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. 2006 Sep 29;188(23):8087–8094. doi: 10.1128/JB.00990-06

TABLE 3.

Kinetic constants of the H. neapolitanus carboxysomal carbonic anhydrase CsoSCA

Fraction Kinetic constant value for:
Hydration reactiona
Dehydration reactionb
kcat (s−1) Km (mM) kcat (s−1) Km (mM)
rCsoSCA (8.9 ± 0.5) × 104 3.2 (± 0.4) (4.6 ± 1.2) × 104 9.3 (± 2.1)
Broken carboxysomes (6.5 ± 0.9) × 104 3.6 (± 0.7) (3.1 ± 0.8) × 104 10.1 (± 3.2)
Intact carboxysomes (1.8 ± 0.2) × 104 12.2 (± 2.3) ND ND
a

The hydration reaction was performed at pH 8.0.

b

The dehydration reaction was performed at pH 7.0. ND, not determined.