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Bacterial strains, plasmids and primers
| Strain, plasmid, or primer | Relevant characteristic(s) or sequence | Source or reference |
|---|---|---|
| Strains | ||
| AG840 | pAG331::cbgA (within cbgA) | This study |
| DK1622 | Wild-type motility and development | (12) |
| Plasmids | ||
| pCR2.1-TOPO | Kanr | Invitrogen |
| pAG331 | 621-bp fragment extending from bp 525 to bp 1146 of the cbgA gene | This study |
| Primers | ||
| OAG106 up | 5′-CCGAAGAAGGCCGAGGACGAG-3′ (amplicon size, 621 bp) | Invitrogen |
| OAG107 down | 5′-GAACTCGGGCGTCAGGAACGTG-3′ |
