TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotype or features | Source or reference |
|---|---|---|
| Escherichia coli B BL21-CodonPlus | ompT gal [dcm] [lon] hsdSB (rB− mB−) λDE3 lysogen pRIL | Stratagene |
| Yersinia enterocolitica 8081 strains (serogroup O:8, American strains)a | ||
| JB580v | ΔyenR (r− m+) pYV+ | 24 |
| YVM619 | Φ(ytxA-lacZYA) | This study |
| YVM707 | ΔytxAB::kan | This study |
| AJD239 | ΔytxR | This study |
| AJD199 | Φ(ytxA-lacZYA) Φ([TnMod-RKm′-lacIqtacp]-ytxR) | This study |
| AJD200 | Φ(ytxA-lacZYA) Φ([TnMod-RKm′-lacIqtacp]-ytxR) | This study |
| AJD254 | ΔytxR Φ(ytxA-lacZYA) | This study |
| AJD378 | ΔytxR Φ(ytxR-lacZYA) | This study |
| AJD1296 | ΔytxR ΔaraGFB::[Φ(ytxA-lacZY)] (Δ110 construct) | This study |
| AJD1297 | ΔytxR ΔaraGFB::[Φ(ytxA-lacZY)] (Δ52 construct) | This study |
| AJD1299 | ΔytxR ΔaraGFB::[Φ(ytxA-lacZY)] (Δ485 construct)b | This study |
| AJD1295 | ΔytxR ΔaraGFB::[Φ(ytxR-lacZY)] (Δ150 construct) | This study |
| AJD1300 | ΔytxR ΔaraGFB::[Φ(ytxR-lacZY)] (Δ500 construct)b | This study |
| AJD1303 | ΔytxR ΔaraGFB::[Φ(ytxR-lacZY)] (Δ86 construct) | This study |
| AJD1304 | ΔytxR ΔaraGFB::[Φ(ytxR-lacZY)] (Δ21 construct) | This study |
| Y. enterocolitica CDC reference strainsc | ||
| 657-83 | Serogroup O:20, American strain | CDC |
| 658-83 | Serogroup O:21, American strain | CDC |
| 655-83 | Serogroup O:18, American strain | CDC |
| 634-83 | Serogroup O:4,32, American strain | CDC |
| 637-83 | Serogroup O:5,27, non-American strain | CDC |
| 661-83 | Serogroup O:27, non-American strain | CDC |
| Y. enterocolitica clinical isolates | ||
| MC5 | Biogroup 1, serogroup O:6,30, Crohn's disease | M. Cafferkey |
| MC7 | Biogroup 1, serogroup O:9, colitis with perforation | M. Cafferkey |
| MC8 | Biogroup 1, serogroup O:9, septicemia | M. Cafferkey |
| MC17 | Biogroup 1, serogroup O:3, acute diarrhea | M. Cafferkey |
| MC22 | Biogroup 3, serogroup O:3, acute appendicitis | M. Cafferkey |
| MC33 | Biogroup 3, serogroup O:3, acute colitis | M. Cafferkey |
| MC28 | Biogroup 4, serogroup O:3, acute diarrhea | M. Cafferkey |
| MC6 | Biogroup 4, serogroup O:3, mesenteric adenitis | M. Cafferkey |
| MC51 | Biogroup 4, serogroup O:3, acute terminal ileitis | M. Cafferkey |
| Other Yersinia strains | ||
| Y. pseudotuberculosis YPIII | pYV+ | 18 |
| Y. pseudotuberculosis K286 | Clinical isolate | 30 |
| Y. kristensenii | Walter Hill, FDAd | |
| Y. frederikensenii | Walter Hill, FDA | |
| Y. frederikensenii MC31 | Clinical isolate (chronic diarrhea, weight loss) | M. Cafferkey |
| Y. rohdei 3022-83 | Dog stool isolate | CDC |
| Y. rohdei 3435-85 | Human stool isolate | CDC |
| Y. aldovae 670-83 | Isolated from water | CDC |
| Y. intermedia | Walter Hill, FDA | |
| Plasmids | ||
| pFUSE | Cmr, mob+ (RP4), R6K ori, lacZYA+ operon fusion vector | 2 |
| pKN8 | BglII linker in SmaI site of pFUSE | 17 |
| pBAD18-Km | Kmr, araBp expression vector, Col E1 ori | 19 |
| pBAD33 | CmraraBp expression vector, p15A ori | 19 |
| pEP185.2 | Cmr, mob+ (RP4), R6K ori | 24 |
| pWSK129 | Kmr, low-copy-number cloning vector, pSC101 ori | 49 |
| pHG329 | Ampr, cloning vector, pBR322 ori | 46 |
| pQE30 | Apr, Col E1 ori, T5p expression vector for His6 fusion proteins | QIAGEN |
| pAJD213 | ΔytxAB::kan in pEP185.2 | This study |
| pAJD593 | araBp-ytxR in pBAD18-Km | This study |
| pAJD610 | ytxA full-length control region in pHG329 | This study |
| pAJD654 | araBp-ytxR in pBAD33 | This study |
| pAJD679 | araBp-His6-ytxR in pBAD18-Km | This study |
| pAJD905 | Cmr, R6K ori, mob+ (RP4), sacB1+, lacZY operon fusion vector | 28 |
| pAJD1060 | Δ110 ytxAp fragment in pAJD905 | This study |
| pAJD1061 | Δ53 ytxAp fragment in pAJD905 | This study |
| pAJD1062 | Δ485 (full length) ytxAp fragment in pAJD905 | This study |
| pAJD1065 | Δ500 (full length) ytxRp fragment in pAJD905 | This study |
| pAJD1057 | Δ150 ytxRp fragment in pAJD905 | This study |
| pAJD1058 | Δ110 ytxRp fragment in pAJD905 | This study |
| pAJD1059 | Δ21 ytxRp fragment in pAJD905 | This study |
| pAJD1252 | ytxR control region (positions −552 to 40) in pWSK129 | This study |
a
All Y. enterocolitica 8081 strains are derivatives of strain JB580v.
b
The Φ(ytxA-lacZY) Δ485 and Φ(ytxR-lacZY) Δ500 constructs represent the full-length control regions (all noncoding upstream DNA) upstream of the lacZY operon.
c
CDC, Centers for Disease Control and Prevention.
d
FDA, Food and Drug Administration.