TABLE 2.
Effects of control region deletions on Φ(ytxA-lacZ) and Φ(ytxR-lacZ) expression
| Control regiona | β-Galactosidase sp actb
|
|
|---|---|---|
| Without YtxRc | With YtxR | |
| Φ(ytxA-lacZ) | ||
| Δ485 | 32 ± 5 | 950 ± 80 |
| Δ110 | 40 ± 4 | 2,120 ± 320 |
| Δ52 | 47 ± 4 | 48 ± 6 |
| Φ(ytxR-lacZ) | ||
| Δ500 | 23 ± 6 | 1,030 ± 340 |
| Δ150 | 310 ± 120 | 2,140 ± 560 |
| Δ86 | 510 ± 60 | 1,810 ± 610 |
| Δ21 | 420 ± 32 | 400 ± 100 |
a
Each strain has a Φ(ytxA-lacZY) or Φ(ytxR-lacZY) fusion integrated on the chromosome with a different amount of DNA upstream of the transcription initiation site, as shown in Fig. 7.
b
β-Galactosidase specific activity was determined as described in Materials and Methods and is expressed in arbitrary (Miller) units. The values are averages± standard deviations.
c
Strains with the araBp vector pBAD33 (without YtxR) or the araBp-ytxR+ expression plasmid pAJD654 (with YtxR) were grown in the presence of 0.2% arabinose as described in Materials and Methods.