Strains |
|
|
Haloarcula sp. strain AS7094 |
Natural host of pSCM201 |
IMCAS |
Haloarcula hispanica strain ATCC 33960 |
Recipient of pSCM201 derivatives |
ATCC |
Escherichia coli JM109 |
recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) F′ [traD36 proAB+lacIq lacZΔM15] |
42 |
Plasmids |
|
|
pUCm-T |
2.77-kb vector for cloning PCR products; Ampr
|
Sangon, China |
pUCmT |
2.77-kb replication type of pUCm-T |
This work |
pBluescript II KS− |
2.96-kb cloning vector; Ampr
|
Stratagene |
pUBP2 |
12.3-kb shuttle vector; Ampr Mevr
|
6 |
pBR322 |
4,361-bp plasmid; Ampr Tetr
|
New England Biolabs |
pSCM201 |
3,463-bp natural plasmid of Haloarcula sp. strain AS7094; GenBank accession no. AY443099
|
This work |
pSCM2011 |
The 3,463-bp HindIII fragment from pSCM201 was cloned into pBluescript II KS−; Ampr
|
This work |
pSCM2012 |
The 3,463-bp ClaI fragment from pSCM201 was cloned into pBluescript II KS−; Ampr
|
This work |
pSCM202 |
The 3,463-bp NcoI fragment from pSCM201 was cloned into pUCmT and then the 3.5-kb Mevr fragment was inserted; Ampr Mevr
|
This work |
pSCM203 |
The 3.5-kb Mevr fragment was cloned into pSCM2012; Ampr Mevr
|
This work |
pSCM204 |
The 3,389-bp PCR product (204F-204R) was cloned into pUCm-T and then the 3.5-kb Mevr fragment was inserted; Ampr Mevr
|
This work |
pSCM212 |
The 2,143-bp PCR product (204F-212R) was cloned into pUCm-T and then the 3.5-kb Mevr fragment was inserted; Ampr Mevr
|
This work |
pSCM213 |
The 2,069-bp PCR product (204F-213R) was cloned into pUCm-T and then the 3.5-kb Mevr fragment was inserted; Ampr Mevr
|
This work |
pSCM214 |
The 1,730-bp PCR product (204F-214R) was cloned into pUCm-T and then the 3.5-kb Mevr fragment was inserted; Ampr Mevr
|
This work |
pSCM216 |
The 1,999-bp PCR product (216F-209R) was cloned into pUCm-T and then the 3.5-kb Mevr fragment was inserted; Ampr Mevr
|
This work |
pSCM217 |
The 1,885-bp PCR product (205F-209R) was cloned into pUCm-T and then the 3.5-kb Mevr fragment was inserted; Ampr Mevr
|
This work |