TABLE 2.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| Haloarcula sp. strain AS7094 | Natural host of pSCM201 | IMCAS |
| Haloarcula hispanica strain ATCC 33960 | Recipient of pSCM201 derivatives | ATCC |
| Escherichia coli JM109 | recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) F′ [traD36 proAB+lacIq lacZΔM15] | 42 |
| Plasmids | ||
| pUCm-T | 2.77-kb vector for cloning PCR products; Ampr | Sangon, China |
| pUCmT | 2.77-kb replication type of pUCm-T | This work |
| pBluescript II KS− | 2.96-kb cloning vector; Ampr | Stratagene |
| pUBP2 | 12.3-kb shuttle vector; Ampr Mevr | 6 |
| pBR322 | 4,361-bp plasmid; Ampr Tetr | New England Biolabs |
| pSCM201 | 3,463-bp natural plasmid of Haloarcula sp. strain AS7094; GenBank accession no. AY443099 | This work |
| pSCM2011 | The 3,463-bp HindIII fragment from pSCM201 was cloned into pBluescript II KS−; Ampr | This work |
| pSCM2012 | The 3,463-bp ClaI fragment from pSCM201 was cloned into pBluescript II KS−; Ampr | This work |
| pSCM202 | The 3,463-bp NcoI fragment from pSCM201 was cloned into pUCmT and then the 3.5-kb Mevr fragment was inserted; Ampr Mevr | This work |
| pSCM203 | The 3.5-kb Mevr fragment was cloned into pSCM2012; Ampr Mevr | This work |
| pSCM204 | The 3,389-bp PCR product (204F-204R) was cloned into pUCm-T and then the 3.5-kb Mevr fragment was inserted; Ampr Mevr | This work |
| pSCM212 | The 2,143-bp PCR product (204F-212R) was cloned into pUCm-T and then the 3.5-kb Mevr fragment was inserted; Ampr Mevr | This work |
| pSCM213 | The 2,069-bp PCR product (204F-213R) was cloned into pUCm-T and then the 3.5-kb Mevr fragment was inserted; Ampr Mevr | This work |
| pSCM214 | The 1,730-bp PCR product (204F-214R) was cloned into pUCm-T and then the 3.5-kb Mevr fragment was inserted; Ampr Mevr | This work |
| pSCM216 | The 1,999-bp PCR product (216F-209R) was cloned into pUCm-T and then the 3.5-kb Mevr fragment was inserted; Ampr Mevr | This work |
| pSCM217 | The 1,885-bp PCR product (205F-209R) was cloned into pUCm-T and then the 3.5-kb Mevr fragment was inserted; Ampr Mevr | This work |