FIG. 2.

³¹P NMR spectra of the final products from incubation at 80°C of reaction mixtures containing A. fulgidus cell extract (50 mg total protein) in 50 mM MOPS buffer (pH 8.1), 5 mM MgCl₂, and 5 mM of the following substrates: CTP plus dl-glycerol-3-P plus l-myo-inositol-1-P (A), CTP plus l-myo-inositol-1-P (B), CDP-inositol plus l-myo-inositol-1-P (C), CDP-glycerol plus l-myo-inositol-1-P (D), and CDP-glycerol plus dl-glycerol-3-P (E). Resonances due to CDP-inositol, CDP-glycerol, DIP, DIPP, DGP, and GPI are labeled. The spectra shown are the differences between the final spectra of the reaction mixtures with the substrates and the spectrum of the control (cell extract with no additions after 1 hour of incubation). The resonances labeled with ○ and □ are due to contaminants in the CDP-glycerol and CDP-inositol preparations, respectively.