FIG. 3.

³¹P NMR spectra showing the conversion of the phosphorylated intermediates into the final products (DGP, GPI, and DIP) by the action of alkaline phosphatase. Panels A, C, and E show the spectra of the reaction mixtures containing cell extract, CTP, NaF, and the following: dl-glycerol-3-P (A), l-myo-inositol-1-P plus dl-glycerol-3-P (C), and l-myo-inositol-1-P (E). After spectral acquisition, samples A, C, and E were treated with alkaline phosphatase, originating from spectra B, D, and F, respectively. The phosphodiester resonances due to DIP (−0.57 ppm), DIPP (−0.38 ppm), GPI (0.60 ppm), GPIP (0.63 ppm), DGP (1.58 ppm), and DGPP (1.51 ppm) are shown.