Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Sep 15;188(23):8118–8127. doi: 10.1128/JB.01044-06

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, American Society for Microbiology

PMC Copyright notice

FIG.4. — Identification of MalE-GadW and MalE-GadX binding sites in gadA and gadBC regulatory regions. DNase I footprinting assays were performed using PgadA (positions −176 to 77) (A) and PgadBC (positions −307 to 77) (B) with different amounts of MalE-GadW and MalE-GadX. Samples were processed as described in Materials and Methods. The protected regions are indicated by vertical lines, and the hypersensitive sites are indicated by asterisks. (C) Nucleotide sequences of the gadA and gadBC regulatory regions. The MalE-GadX and MalE-GadW binding sites, identified by DNase I footprinting assays (panels A and B), are indicated by shading, and the hypersensitive sites are indicated by asterisks. The low-affinity MalE-GadX site in the gadBC promoter (37) is underlined with a dashed line. The H-NS binding sites in the gadA promoter (14) are underlined with dotted lines. The −35 and −10 hexamers are enclosed in boxes. The GAD box is underlined with a boldface line in both promoter regions.

FIG.4. — Identification of MalE-GadW and MalE-GadX binding sites in gadA and gadBC regulatory regions. DNase I footprinting assays were performed using PgadA (positions −176 to 77) (A) and PgadBC (positions −307 to 77) (B) with different amounts of MalE-GadW and MalE-GadX. Samples were processed as described in Materials and Methods. The protected regions are indicated by vertical lines, and the hypersensitive sites are indicated by asterisks. (C) Nucleotide sequences of the gadA and gadBC regulatory regions. The MalE-GadX and MalE-GadW binding sites, identified by DNase I footprinting assays (panels A and B), are indicated by shading, and the hypersensitive sites are indicated by asterisks. The low-affinity MalE-GadX site in the gadBC promoter (37) is underlined with a dashed line. The H-NS binding sites in the gadA promoter (14) are underlined with dotted lines. The −35 and −10 hexamers are enclosed in boxes. The GAD box is underlined with a boldface line in both promoter regions.