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. 2006 Sep 15;188(23):8118–8127. doi: 10.1128/JB.01044-06

FIG. 6.

FIG. 6.

Effect of gadW mutation and GadW overexpression on gadAX expression. (A) Northern blot analysis of total RNA extracted from E. coli wild-type strain MC4100 (+) and mutant MC4100gadW (−) grown in LB-MES at pH 5.5 to the exponential phase (E) (OD600, ∼0.5) and stationary phase (S) (OD600, ∼2). Aliquots (10 μg of total RNA) were subjected to electrophoresis, transferred onto nylon filters, and hybridized with the gadX-specific probe (37). The sizes of reference rRNAs are indicated on the right. The positions of the gadX and gadAX transcripts are indicated by arrows on the left. (B) Northern hybridization analysis of total RNA extracted from IPTG-induced E. coli JM109 cultures carrying the pQE60 and pQEgadW plasmids. Cells were grown in LB at pH 7.4 until the mid-exponential phase (OD600, ∼1). Aliquots (10 μg of total RNA) were subjected to electrophoresis, transferred onto nylon filters, and hybridized with the gadX-specific probe (37). The sizes of reference rRNAs are indicated on the right. The position of the gadAX transcript is indicated by an arrow on the left.