TABLE 2.

Oligonucleotides used in this study

Oligonucleotide	Sequencea	Restriction site
Wfor(−201)	5′-GGGAATTCATAAACATAAGCTATACGCTG-3′	EcoRI
Wfor(−14)	5′-GGGAATTCTATAAGGGATAATCATGACT-3′	EcoRI
Wfor(+1)	5′-GGCATATGACTCATGTCTGCTC-3′	NdeI
Wrev(+731)	5′-CCGGATCCTGTCAGGAAAAGGTACC-3′	BamHI
Wrev(+879)	5′-GGGGATCCAAACATGCCGAGTCTTTTCC-3′	BamHI
PgadB-307for	5′-GGGGATCCGGGGATTTTATCAATAATCGC-3′	BamHI
PgadBrev	5′-GGAAGCTTCGTGAATCGAGTAGTTCCGAC-3′	HindIII
PCgadArev	5′-CCGGATCCATTTCGAACTCCTTAAA-3′	BamHI
PCgadBrev	5′-CCGGATCCATCCATTTTAAACTCCTTAA-3′	BamHI
PCgadAforH	5′-CCAAGCTTCCGTACTTCCATCGCG-3′	HindIII
PCgadAforB	5′-CCAGATCTCCGTACTTCCATCGCG-3′	BglII
PCgadBforH	5′-GGAAGCTTCGCTATTTTTATGTAATAATTT-3′	HindIII
PCgadBforB	5′-GGAGATCTCGCTATTTTTATGTAATAATTT-3′	BglII
Xfor(−221)	5′-GATATATGTTATATGAATGTTTA-3 ′
Xrev(+854)	5′-CTATAATCTTATTCCTTCCG-3′
ΔgadEfor	5′-AGCAAGTTATGATTTTTCTCATGACGAAAGATTCTTGTGTAGGCTGGAGCTGCTGCTTC-3
ΔgadErev	5′-CTAAAAATAAGATGTGATACCCAGGGTGACGATGTCGCATTCCGGGGATCCGTCGACC-3′
rRNA16Sfor	5′-CGTTACCCGCAGAAGAAGC-3′
rRNA16Srev	5′-GTGGACTACCAGGGTATCTAATCC-3′
gadA/Brev	5′-GGCGCATTCCACAGATCG-3′
gadBrev	5′-TTGTCGATCCAGTTTTTGTTAATG-3′
gadBfor	5′-GGAGTTTAAAATGGATAAGAAGCAAG-3′
gadXrev	5′-CTTATTCTGCGATAGTTGCG-3′
gadXfor	5′-AATATTAAGTCAACTATGCAATCACTAC-3′

^aRestriction sites are underlined.