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The M. tuberculosis sigM locus and mutant construction. A. sigM is located 5′ of the thioredoxin reductase-thioredoxin operon. Our sequence data indicate a 93-bp intergenic region between sigM and Rv3912 in H37Rv, identical to the CDC1551 genome sequence but distinct from the published H37Rv sequence (9, 17). B. Southern blot analysis demonstrates the insertional inactivation of sigM. Wt, H37Rv; M−, sigM mutant, C, complemented sigM mutant.
