FIG. 3.

Primer extension analysis of SigM-dependent promoters. RNA was isolated from the sigM overexpression strain (M+) and the sigM mutant (M−) and reverse transcribed with gene-specific antisense primers. A sequencing reaction was performed with the same primer, and reactions were resolved on sequencing gels to identify transcription start sites. Reverse transcription was performed using the same RNA with a sigA primer as a control in each experiment.