FIG. 5.

sigM expression represses PDIM biosynthetic gene transcription. A. Quantitative primer extension analysis was performed using equal amount of total RNA from the H37Rv (wt), sigM mutant (M−), and sigM overexpression (M+) strains of M. tuberculosis. The numbers below each lane indicate quantification of the transcript relative to expression in the wt, normalized to sigA expression in the same experiment. Experiments were performed twice, and representative results are shown. B. Organization of the PDIM biosynthesis and transport locus. Arrows indicate the three transcripts previously identified in this region (5).