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Southern blot hybridizations of B. pertussis, B. holmesii, and B. avium with 16S rRNA gene probes. Genomic DNA from each of the three strains was restriction digested with ClaI or NcoI as indicated and hybridized to a B. pertussis-specific probe (left panel) and a broad-range 16S rRNA gene probe (B-16S8F [right panel]) (15). Biotinylated DNA markers are in the first lane, and numbers at the left are fragment sizes (in base pairs).
