FIG. 1.

Homologous recombination at the MgPa operon in M. genitalium. (A and D) Schematic representation of the two possible crossover events of the pΔmg191 and pΔmg192 deletion constructs with the MgPa operon. (B and E) mg191 and mg192 genes, respectively, disrupted by double-crossover recombination. (C) mg191 gene disrupted by single-crossover recombination through the downstream flanking region. Hatched boxes represent flanking regions of the mg191 (lines from right to left) and mg192 (lines from left to right) genes in suicide plasmids pΔmg191 and pΔmg192, respectively. Black, tetM438 selectable marker; white, mg191 and mg192 genes; lines inside genes, probes S1, S2, and S3. The sizes of fragments resulting from HindIII (H) and BglII (Bg) digestions of the WT and each recombinant mutant DNA are also indicated.