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. 2006 Oct 6;188(24):8627–8637. doi: 10.1128/JB.00978-06

FIG. 4.

FIG. 4.

Western blot analyses of the different mutants and the WT strain. Blots were probed with an anti-P140 monoclonal antibody (A and B) or anti-P110 polyclonal antibody (C and D). In panels A and C, lanes 1 and 2 correspond to class II and class I mutants, respectively. In panels B and D, lanes 1 and 2 correspond to class II and class I mutants transformed with pTnTetMG191-192, respectively. Lanes 3 and 4, class II and class I mutants transformed with pTnTetMG192, respectively; lanes 5 and 6, are class II and class I mutants transformed with pTnTetMG191, respectively; lanes 7 and 8, mg191− mutant clone 4 and mg192− mutant clone 5, respectively; lane 9, WT strain. The arrows in panel B indicate the P140 doublet, while those in panel D indicate the presence of low quantities of P110.