FIG. 5.

Polysaccharide profiles produced by the wild type and nodPQ- and cysH-deficient strains of M. loti during size-exclusion chromatography. Water layer extracts were prepared as described in Materials and Methods and then chromatographed on Sephadex G-150 in detergent (dissociative conditions). The eluants were monitored by refractive index detection from M. loti NZP-2235 (wild type) (A), the M. loti nodPQ mutant (B), and the M. loti cysH mutant (C). (Inset) Prior to chromatography, polysaccharide extracts were analyzed by deoxycholate-PAGE and stained sequentially with Alcian blue, periodate, and silver reagent. KPS I and KPS II comigrate on this gel system.